TABLE 1.
Summary of results from Nanopore sequencing based on pooled samples with various titers of influenza A virus and a consistent titer of Hazara virus controla
| Influenza A virus titer (genome copies/ml) | Dilution pool no. | Total no. of reads | No. of influenza A virus reads (reads per million) | Influenza A virus subtypingb | No. of Hazara virus reads (reads per million) |
|---|---|---|---|---|---|
| 106 | 1 | 473,718 | 33,103 (6.9 × 104) | H3N2 | 527 (1.1 × 103) |
| 2 | 572,106 | 6,957 (1.2 × 104) | H3N2 | 102 (178) | |
| 3 | 526,852 | 41,196 (7.8 × 104) | H3N2 | 534 (1.0 × 103) | |
| 104 | 1 | 354,163 | 280 (791) | H3N2 | 738 (2.1 × 103) |
| 2 | 433,033 | 299 (690) | H3N2 | 691 (1.6 × 103) | |
| 3 | 43,512 | 2 (46) | Not possible | 9 (207) | |
| 103 | 1 | 231,929 | 7 (30) | H3N2 | 298 (1.3 × 103) |
| 2 | 461,281 | 24 (52) | H3N2 | 638 (1.4 × 103) | |
| 3 | 397,672 | 2 (5) | Not possible | 38 (96) | |
| 102 | 1 | 375,183 | 1 (3) | Not possible | 453 (1.2 × 103) |
| 2 | 671,133 | 0 (0) | Not possible | 598 (891) | |
| 3 | 37,897 | 0 (0) | Not possible | 2 (53) | |
| Negative | 1 | 903,430 | 0 (0) | NA | 1,731 (1.9 × 103) |
| 2 | 900,471 | 0 (0) | NA | 692 (768) | |
| 3 | 818,549 | 0 (0) | NA | 54 (66) |
a
Each dilution is undertaken in triplicate (shown as 3 dilution pools).
b
NA, not applicable.