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. 2019 Dec 23;7:342. doi: 10.3389/fcell.2019.00342

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Copyright © 2019 Gao, Zhang, Wei, Dai, Zhang, Yang, Li and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Notch pathway inhibition by miR-128-3p decreases the expression of neurocyte markers in neurogenic differentiation. (A) Immunofluorescence staining illustrates activation of the Notch pathway based on the nuclear expression of intercellular activated notch 1 (Notch intracellular domain: NICD) and Hes 5 after ATRA induction and miR-128 addition. (B) Fluorescence intensity was analyzed using ImageJ tools. (C,D) Western blot data of NICD and HES5 expression after JAG1, BMS-708163, or miR-128 overexpression treatment. Protein abundance was analyzed using ImageJ tools. ^∗P < 0.05; ^∗∗P < 0.01; ns, non-significant. (E) Neurocyte markers were tested using flow cytometry in induced AECs exposed to 708163 (an inhibitor of the Notch pathway) or Jagged 1 (activator for the Notch pathway). iAECs, induced AECs.