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. 2019 Dec 23;7:342. doi: 10.3389/fcell.2019.00342

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Copyright © 2019 Gao, Zhang, Wei, Dai, Zhang, Yang, Li and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATRA increases the expression of MEG3 through the cAMP/CREB pathway to modulate neurogenic differentiation. (A) A siRNA for MEG3 (si-MEG3) effectively depressed the expression of MEG3 in induced AECs (P < 0.05). (B) The concentration of cAMP was detected between normal significantly increased in induced compared with normal AECs (P < 0.05). (C) Expression of cAMP response element-binding protein (CREB) was detected under ATRA or 666-15 (a CREB inhibitor) treatment using immunofluorescence staining. (D) MEG3 expression was analyzed under 666-15 or Jagged 1 treatment by RT-qPCR. (E,F) Expression of β III-tubulin and GFAP were as assessed using Western blotting. (G) β III-tubulin and GFAP expression was analyzed following single or assorted overexpression of MEG3 (o-MEG3), si-MEG3 or 666-15 treatment in induced AECs. iAECs, induced AECs; siMEG3-NC, siMEG3 negative control. ^∗P < 0.05; ^∗∗P < 0.01; ns, non-significant.