Synthesis and
characterization of protein-adsorbed SNAs. (A) Schematic
representation of monoclonal antibody (top) or IgG (bottom) immobilization
on the ODN shell of SNAs. (B) Distributions of the hydrodynamic diameters
of bare, IgG- (IgG@SNA), anti-HER2- (HER2@SNA), and HSA-immobilized
SNAs (HSA@SNA). (C) ζ potentials of bare SNAs, IgG@SNAs, HER2@SNAs,
and HSA@SNAs. (D) Schematic depicting the displacement of Texas Red-X-labeled
proteins from the surface of Cy5-labeled SNAs by serum proteins. The
fluorescence of Texas Red-X increases as protein displacement occurs.
(E) Kinetic fluorescence profiles of Texas Red-X-labeled IgG, anti-HER2,
and HSA during incubation with 10% serum proteins.