Figure 4.

Fixed platelets bind to and roll on VWF-coated surface under flow. (A) Footprints of platelets rolling on VWF-coated surface under 20 dyn cm⁻² wall shear in 150 mM and 500 mM NaCl in the first 8 seconds of flow. Brighter patterns indicate overlapped footprints. The accumulated number of platelets bound to the surface (B) and the average speed of platelet rolling on the surface (C) are plotted as a function of wall shear stress. The inset shows a zoom-in of the rolling speed between 0.5 and 10 dyn cm⁻² for easier viewing. For shear stresses lower than 0.2 dyn cm⁻² and higher than 50 dyn cm⁻² in 500 mM NaCl buffer, fewer than 3 platelets bound to the surface and the rolling speed was not plotted. Error bars indicate standard errors of the mean.