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. 2019 Dec 29;17:110. doi: 10.1186/s12915-019-0720-y
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Additional file 9: Movie S1. Z-stack for one example Tg(ubi:nls-GCaMP6f)m1300 fish. This movie shows a z-stack of a Tg(ubi:nls-GCaMP6f)m1300 larvae at 5 dpf imaged under the above mentioned setup (except using a × 20/1.0 Zeiss objective) resulting in an imaged area of 450.56 × 450.56 μm in x and y with 0.88 μm per slice in z. The movie is contrast enhanced and imaged with increased laser power (roughly 33 mW after the objective) to highlight GCaMP6f expression (same fish as in Fig. 1b).