Skip to main content
. Author manuscript; available in PMC: 2021 Jan 30.
Published in final edited form as: J Pharm Biomed Anal. 2019 Sep 30;178:112884. doi: 10.1016/j.jpba.2019.112884

Figure 2. Optimization of Chromatography Conditions for D-(+)-Glucose-PMP.

Figure 2.

A) Representative total ion current (TIC) chromatogram of D-(+)-Glucose-PMP 12.48μg/mL in C18 column, Inj. Volume: 10μL, showing very low chromatographic sensitivity at retention time 2.1 min compared to PMP alone (large PMP peak shown in box at upper right-hand corner) at 10 min. B) Extracted D-(+)-Glucose-PMP showed very low chromatographic area at 2.1 min and poor resolution. C) Extracted PMP has very high chromatographic area at retention time 10 min and can be detected easily over D-(+)-Glucose-PMP in C18 column, indicating unsuitability of this column for identification of D-(+)-Glucose-PMP. D) TIC chromatogram of D-(+)-Glucose-PMP 50μg/mL in NH2P-40 3E column, Inj. Vol: 10μL, shows D-(+)-Glucose-PMP detection with distinguishing chromatographic peak at retention time 3 min in NH2P-40 3 E column. E) Extracted chromatogram of D-(+)-Glucose-PMP in NH2P-40 3E column at m/z 510.2 shows sele.ctive D-(+)-Glucose-PMP at retention time 3.5 min.