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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Oct 8;520(2):341–346. doi: 10.1016/j.bbrc.2019.10.032

Figure 3. Ighmbp2 protein levels in the spinal cord and the gastrocnemius NMJ of the FVB-nmd mice are severely affected compared to “HET”.

Figure 3.

(A) Western blot of the spinal cord extracts of the FVB-nmd at P14 (n=7) demonstrates a considerable decrease in Ighmbp2 protein levels compared to the age-matched “HET” littermates (n=4) (two-tailed t test P<0.0001). (B) NMJs from gastrocnemius muscle of FVB-nmd mice at P14 compared to those of age-matched “HET’ littermates. Teased fibers were labeled with α-BTX for acetylcholine receptors (ACHRs), anti-neurofilament, and anti-vesicular acetylcholine transporter (VAChT) for nerve terminals. NMJs of the FVB-nmd are mostly denervated as opposed to the “HET” that shows no sign of denervation.