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. 2019 Nov;16(4):771–783. doi: 10.20892/j.issn.2095-3941.2019.0050

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(A and B) Two short hairpin RNAs were used to knock down ILF2 expression in H446 and H82 cells. RT-qPCR and Western blot were used to confirm the knockdown efficiency. (C and D) H446 and H82 cells infected with shILF2 or scrambled shRNA were assessed by colony formation assay. Representative graphs showing the colony formation capacity of H446 cells are shown in panel (C). (E) H446 and H82 cells infected with shILF2-1, shILF2-2 or scrambled shRNA were analyzed by BrdU incorporation assays. (F and G) RT-qPCR and Western blot were used to assess ILF2 expression levels in H446 and H82 cells infected with either an ILF2-overexpressing or a vector control lentivirus. (H and I) H446 and H82 cells infected with ILF2-overexpressing or vector control lentivirus were assessed by colony formation assay. Representative graphs showing the colony formation capacity of H446 cells are shown in panel (H). (J) H446 and H82 cells infected with ILF2-overexpressing or control vector lentivirus were analyzed by BrdU incorporation assays. (K, L and M) Female nude mice were injected with H446-scramble or H446-shILF2-1 cells (5 × 10<sup>6</sup> cells/animal). Three weeks after injection, the mice were euthanized. Tumor burden was evaluated based on tumor volumes and weights (<italic>n</italic> = 8). (N) Xenograft tumor sections were stained with H&amp;E or an anti-Ki67 antibody. *, <italic>P</italic> &lt; 0.05, **, <italic>P</italic> &lt; 0.01.

ILF2 promotes SCLC cell proliferation and tumorigenesis.