1.

The clinical significance of Anxa2 in cancers

Tumor type	Sample	Sample size	Expression	Significance	Reference
Breast cancer	Tissue	IDC* = 100 DCIS** = 20 Normal = 50	mRNA	Upregulated in IDC Lymph node invasion	6
	Tissue	n = 20	Protein	Upregulated in cancer	7
	Tissue	n = 85	Protein	Upregulated in invasive cancer Advanced stage High histological grade High EGFR expression Poor OS and DFS	8
	Blood Tissue	Cancer = 40 Normal = 40 n = 274	Protein	Upregulated in cancer High histological grade High Ki-67 proliferation index P53 overexpression ER- subtype TNBC subtype	9
	Blood Tissue	n = 36 n = 45	Protein	Upregulated in HER2- cancer TNBC subtype	10
	Blood Tissue	n = 101 n = 101	mRNA Protein	Upregulated on CTCs*** Upregulated in tumor with EMT CTCs ER- subtype Bcl-2-	11
Gynecologic tumors
Ovarian cancer	Tissue Public database	n = 109 n = 1604	Protein mRNA	Upregulated in cancer Poor RFS and OS	14
	Tissue	n = 119	Protein	Upregulated in cancer Advanced FIGO stage High histologic grade Ascites Malignant tumor cells in peritoneal fluid Larger residual tumor size High Ki-67 expression Poor OS	15
Endometrial cancer	Tissue	Cancer = 84 Atypical hyperplasia = 30 Normal = 18	Protein	Upregulated in cancer Advanced FIGO stage Poor differentiation Myometrial invasion Lymph node invasion Poor OS	16
	Tissue	n = 53	Protein	Upregulated in lymph node metastasis cases	17
	Tissue	n = 293	Protein	Upregulated in recurrence cases Upregulated in NEEC than EEC**** Poor RFS	18
Cervical cancer	Tissue	Cancer = 33 Normal = 17	Protein	Upregulated in cancer	19
	Tissue	Cancer = 336 Normal = 46	Protein	Upregulated in squamous cell carcinoma Advanced stage Larger tumor size Stromal invasion depth Lymph node invasion Parametrial involvement Poor DFS and OS	20
	Tissue	n = 68	Protein	Upregulated in post-chemotherapy tissue Overexpression in tumor cells in chemotherapy non-response group Stroma Overexpression correlates with poor PFS	21
Digestive system tumors
Pancreatic cancer	Tissue	Cancer = 21 Pancreatitis= 9 Normal = 10 Cancer = 51 Pancreatitis= 32 Normal = 30	mRNA Protein	Upregulated mRNA level in cancer than in normal tissues (non-significant elevation than in pancreatitis) Cell surface redistribution of Anxa2 protein from PanIN-1 lesions to cancer	22
	Tissue	n = 78	Protein	Predominant cancer cell surface location High expression with TNC overexpression in advanced stage, hematogenous and peritoneal recurrence cases Poor DFS and OS	23
	Tissue	n = 56	Protein	High stromal expression correlates with poor DFS and OS	24
	Tissue	n = 62	Protein	Upregulated in cancer Gemcitabine resistance Poor DFS and OS	25
Gastric cancer	Tissue	n = 126	mRNA, protein	Upregulated in cancer Advanced stage Lymph node metastasis Distant metastasis	26
	Tissue	Cancer = 51 Normal = 24	Protein	Upregulated in cancer Advanced stage Lager tumor size Lymph node metastasis Poor differentiation	27
Hepatocellular cancer	Tissue	Cancer = 34 Hepatitis = 39 Normal = 5	mRNA, protein	Upregulated in cancer (negative in normal, low expression in hepatitis, high expression in cancer) Poor differentiation of tumor	28
	Tissue Blood	Cancer = 224 Adjacent normal tissues = 224 Cancer = 175 Hepatitis = 23 Cirrhosis = 51 Benign tumors = 19 Normal = 49	Protein	Upregulated in cancer Upregulated in serum of HCC patients than in hepatitis, cirrhosis, benign tumors and healthy groups Upregulated in serum in AFP-negative HCC patients	30
	Blood	Cancer = 50 Chronic liver disease = 25 Normal = 15	Protein	Upregulated in cancer than in chronic disease and normal groups	29
	Tissue	n = 105	Protein	Poor RFS and OS	31
Colorectal cancer	Tissue	n = 105	Protein	Histologic type Lager tumor size Invasion depth Advanced stage Poor OS	32
	Tissue	Primary cancer = 59 Liver metastasis = 8	Protein	Upregulated in cancer Marked increase in advanced tumor and metastatic lesions	33
	Tissue	LNM***** = 45 Non-LNM = 45	Protein	Upregulated in lymph node metastasis cancer	34
Lung Cancer	Tissue	Cancer = 71 Normal = 20	Protein	Upregulated in cancer Advanced stage Lymph node metastasis Poor OS	35
	Tissue Public database	Cancer = 152 Normal = 36 n = 2437	Protein mRNA	Upregulated in cancer Advanced stage DDP resistance Poor OS	36
	Tissue	n = 36	Protein	Upregulated in squamous carcinomas	37
Head and neck tumors
Glioma	Tissue Public database	n = 89 n = 710	Protein mRNA	Upregulated in cancer Overexpression in aggressive tumors Poor PFS and OS	38
	Tissue	Cancer = 130 Normal = 15	mRNA Protein	Upregulated in cancer High histological grade Poor OS	39
	Tissue Public database	Cancer = 99 Normal = 12 n = 923	mRNA	Anxa2 and its pseudogene upregulated in cancer Poor OS	41
Pediatric neuroblastoma	Tissue	n = 42	Protein	Advanced stage More chemotherapy cycles Metastasis Poor OS	42
Oral squamous cell carcinoma	Saliva	Cancer = 131 OPMD******= 233 Normal = 96	Protein	Upregulated in cancer	44
	Serum	Cancer = 126 Benign disease = 115 Normal = 158	Protein	Upregulated in cancer Poor OS	45
	Tissue	n = 30	Protein	Upregulated in cancer Well differentiation	46
	Tissue	n = 106	Protein	Larger tumor size Less recurrence Well differentiation	47
Laryngeal cancer	Tissue	Cancer = 209 Normal = 88	Protein	Upregulated in cancer Larger tumor size Advanced stage Lymph node metastasis Distant metastasis Poor OS	48
Nasophary-ngeal cancer	Tissue	Primary cancer = 48 Metastatic Cancer = 13	Protein	Metastasis Poor OS	50
Genitourinary system tumors
Renal cancer	Tissue	n = 47 n = 13	mRNA Protein	Upregulated in cancer	51
	Tissue	Primary cancer = 18 Primary cancer = 154 Metastatic tumor = 24	mRNA Protein	Upregulated in cancer Highly expressed in metastatic tumor Advanced stage Higher nuclear grade Poor MFS	52
Bladder cancer	Tissue	n = 60	Protein	Upregulated in cancer Advanced stage Higher expression in short term recurrence group	53
Prostate cancer	Tissue	Cancer = 85 Benign hyperplasia = 40	Protein	Downregulated in cancer Less metastasis Early stage Better OS	54
	Tissue	Cancer = 31 Benign hyperplasia = 12 Normal = 16	Protein	Downregulated in cancer	55
	Tissue	n = 24	Protein	High expression in relapse group	56
	Tissue	n = 87	Protein	Re-expression in poorly differentiated adenocarcinoma	57
Hematologic tumors
Acute promyelocytic leukemia	Blood Bone marrow	n = 14	Protein	Upregulated in APL	60
Acute myelogenous leukemia	Blood	n = 103	Protein	Highest expression in M3 cells and downregulated after As2O3 treatment with hyperfibrinolysis reduction	61
Acute lymphoblastic leukemia	Blood Bone marrow	Prednisolone-resistant = 24 Prednisolone-sensitive = 19	mRNA Protein	Higher expression and phosphorylation in prednisolone-resistant group	62
* IDC = invasive ductal cancer; ** DCIS = ductal carcinoma in situ; *** CTC = circulating tumor cells; **** EEC = endometrioid endometrial carcinomas; ***** LNM = lymph node metastasis; ****** OPMD = oral potentially malignant disorders.