KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-T7 mouse	EMD Millipore	#69522-3; RRID: AB_11211744
Anti-cleaved caspase-3 rabbit	Cell Signaling	#9661L; RRID: AB_2341188
Anti-TRA2β rabbit	Abcam	#AB31353; RRID: AB_778565
Anti-β Tubulin III rabbit	Genescript	#A01203-40; RRID: AB_1289179
anti-SRSF4	Bethyl Labs	#A303-670A; RRID: AB_11204752
anti SRSF6	CSHL antibody Facility	AK9-156
anti-SRSF9	CSHL antibody Facility	#AK251-24
Anti-ki67 rabbit	Zymed/ Invitrogen	#18-0191Z
Alexa Fluor 568 anti-mouse	Invitrogen	#A-11031; RRID: AB_144696
Alexa Fluor 488 anti-rabbit	Invitrogen	#A-11034; RRID: AB_2576217
IRDye 800CW Goat anti-Rabbit IgG (H + L)	Li-Cor	#926-32211; RRID: AB_621843
IRDye 680 Goat anti-Mouse IgG (H + L)	Li-Cor	#926-3220
Chemicals, Peptides, and Recombinant Proteins
Matrigel Growth Factor Reduced	BD /Corning	#354230
Human Epithelial Growth Factor	Peprotech	#100-15
4-hydroxy-tamoxifen	Sigma	#H7904-5mg
Collagen	BD /Corning	#354236
Cell Recovery Solution	BD /Corning	#354253
Puromycin dihydrochloride	Sigma	#P8833
Hygromycin B	Sigma	#400053
G418	Goldbio	#G-418
Insulin	Sigma	#I-1882
D-luciferin, potassium salt	Goldbio	#LUCK-10 g
Doxycycline	RPI corporation	#D43020
Doxycycline food pellets	Harlan Teklad	#TD.05125
Critical Commercial Assays
RNAeasy kit	QIAGEN	#74106
TrueSeq stranded mRNA Kit	Illumina	#RS-122-2101
iTaq Universal SYBR green Supermix	Bio-Rad	#172-5121
MTT assay	ATCC	# 30-1010K
Deposited Data
RNA-seq MCF-10A	This paper	GSE137440
RNA-seq MDA-MB231	This paper	GSE137408
Experimental Models: Cell Lines
MCF-10A	Laboratory of Senthil Muthuswamy	N/A
MDA-MB231	Laboratory of Min Yu	N/A
SUM159PT	Laboratory of Steve Ethier	N/A
Recombinant DNA
PWZL-T7-SRSF1-Hygromycin	(Anczuków et al., 2012)	N/A
PWZL-T7-SRSF2-Hygromycin	This paper	N/A
PWZL-T7-SRSF3-Hygromycin	This paper	N/A
PWZL-T7-SRSF4-Hygromycin	This paper	N/A
PWZL-T7-SRSF6-Hygromycin	This paper	N/A
PWZL-T7-SRSF9-Hygromycin	This paper	N/A
PWZL-T7-HNRNPA1-Hygromycin	This paper	N/A
PWZL-T7-TRA2B-Hygromycin	This paper	N/A
pBABE-MYC.ER-Puromycin	(Eilers et al., 1989)	N/A
TRMPV-Neo-SRSF4-sh1	This paper	N/A
TRMPV-Neo-SRSF4-sh2	This paper	N/A
TRMPV-Neo-SRSF6-sh1	This paper	N/A
TRMPV-Neo-SRSF6-sh2	This paper	N/A
TRMPV-Neo-SRSF9-sh1	This paper	N/A
TRMPV-Neo-SRSF9-sh2	This paper	N/A
TRMPV-Neo-TRA2B -sh1	This paper	N/A
TRMPV-Neo-TRA2B-sh2	This paper	N/A
TRMPV-Neo-HNRNPA1-sh1	This paper	N/A
TRMPV-Neo- HNRNPA1-sh2	This paper	N/A
TRMPV-Neo- control-sh1	This paper	N/A
rTTA3-Puro	(Zuber et al., 2011)	N/A
Sequence-Based Reagents
shRNA sequence	This paper Table S7A	N/A
QPCR primer sequences	This paper Table S7B	N/A
RT-PCR primer sequences	This paper Table S7C	N/A
Software and Algorithms
Cbio Portal	(Cerami et al., 2012)	www.cbioportal.org
ISB-CGC	(Reynolds et al., 2017)	https://isb-cgc.appspot.com/
dockerhub	N/A	https://hub.docker.com/
STAR v.2.5.1b	(Dobin et al., 2013)	https://github.com/alexdobin/STAR
Cufflinks 2.2.1	(Trapnell et al., 2012)	http://cole-trapnell-lab.github.io/cufflinks/
BedTools v.2.25.0	(Quinlan and Hall, 2010)	https://bedtools.readthedocs.io/en/latest/
Trimmomatic v.0.36	(Bolger et al., 2014)	http://www.usadellab.org/cms/?page=trimmomatic
rMATS v.3.2.5	(Shen et al., 2014)	http://rnaseq-mats.sourceforge.net/
enrichR	(Chen et al., 2013; Kuleshov et al., 2016)	https://cran.r-project.org/web/packages/enrichR/vignettes/enrichR.html
SpliceCore®	Envisagenics	https://www.envisagenics.com/platform/
Prognoscan database	(Mizuno et al., 2009)	http://gibk21.bse.kyutech.ac.jp/PrognoScan/index.html
Axiovision Digital Image Processing Software	Carl Zeiss	N/A
GraphPad Prism	GraphPad	N/A
ImageJ Digital Image Processing Software	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
Photoshop and Illustrator CC2018	Adobe	N/A