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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Behav Brain Res. 2019 Sep 13;378:112237. doi: 10.1016/j.bbr.2019.112237

Fig. 4.

Fig. 4.

Low magnification photomicrographs illustrating FosB/ΔFosB and tryptophan hydroxylase (Tph) immunostaining from different rostrocaudal levels of the dorsal raphe nucleus (DR) in Experiment 2. FosB/ΔFosB immunostaining can be identified by a dark blue/black nuclear stain. Tryptophan hydroxylase-immunoreactive (ir) neurons and dendrites can be identified by a red/brown cytoplasmic stain. The rostrocaudal levels selected for analysis were (A) −7.64 mm bregma, (B) −8.18 mm bregma, and (C) −8.54 mm bregma. The subdivisions of the DR that were analyzed at each rostrocaudal level are delineated by dashed lines. Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL/VLPAG, dorsal raphe nucleus, ventrolateral part/ventrolateral periaqueductal gray; mlf, medial longitudinal fasciculus. Scale bar, 500 μm.