Fig. 2.

Brain lesion profile and PrP^res Western blot in the brain of tgARQ and tg338 mice inoculated with AS scrapie adapted in tgBov. Groups of mice (n ≥ 6) that express ovine VRQ PrP (tg338 mice) or ovine ARQ PrP (tgARQ mice) were intracerebrally challenged with atypical scrapie isolates (AS) or an ovine c-BSE isolate that had previously been adapted (2 iterative passages) in tgBov mice. (A) After 2 iterative passages in each mouse line, a standard lesion profile was established by scoring the vacuolar changes observed in predefined brain areas. In AS graphs, △, AS 2; ▽, AS 3. (B) The Western blot profile of PK-resistant PrP (PrP^res) in the original AS isolates and in the brain of inoculated mice was established by Western blot using anti PrP monoclonal antibody Sha31 (epitope 145-YEDRYYRE-152). The same Western blot PrP^res control (classical scrapie isolate) was used on all of the gels labeled as WB control.