Fig. 2.

Efficient deletion of Paneth cells in the pLys^DTR KI mice on DT administration. In situ hybridization of cryptdin-1 (A and B), lysozyme-specific immunostaining (C and D), and confocal imaging for dsRED expression (E and F) on jejunum sections from the untreated pLys^DTR KI (A and C) and pLys^dsRED (E) control mice and the pLys^DTR KI (B and D) and pLys^DTR_ pLys^dsRED (F) mice DT-treated for 6 consecutive days, at 16 h after the last DT injection. The analysis shows the absence of Paneth cells in the intestines of DT-treated pLys^DTR mice (B and D) and pLys^DTR_pLys^dsRED mice (F), in contrast to the controls (A, C, and E). (Scale bars: 100 µm in A–D; 50 µm in E and F.)