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. 2019 Dec 5;116(52):27124–27132. doi: 10.1073/pnas.1907500116

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Copyright © 2019 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 3. — Stay-green phenotype, photosynthesis, and drought. (A) Two LHC PSII genes (LHCBs) (Sobic.003G209800 and Sobic.003G209900) show stronger down-regulation in RTx430 than BTx642 under postflowering drought stress. (B) Immunoblot analysis of representative subunits of photosynthetic complexes PSI (PsaA), PSII (D1 and CP47), and cytochrome b₆f complex (Cyt b₆) under control and 3 wk postflowering drought (week 11) with ATP synthase (AtpB) as a loading control (additional replicate is in SI Appendix, Fig. S15). (C) Proline levels determined on fresh weight (FW) leaf samples via a ninhydrin spectrophotometric assay for both drought conditions and genotypes. (D) Average value of 3 HPLC assays of total chlorophyll levels (y axis) plotted against week (x axis) showing constitutive differences between BTx642 and RTx430 after flowering and a sharp increase in chlorophyll levels under preflowering drought of RTx430; bars indicate ±SD intervals. (E) Bulk GST enzymatic activity in leaf extracts (y axis) at 3 timepoints (x axis). (F) Heatmap of centered log2 difference between the constitutive gene-expression values of RTx430 and BTx642 for the 68 expressed GST genes.