Fig. 3.

Manipulations of PNNs in adult mice affected long-term contextual fear memory reconsolidation. (A, Top) Schematic drawing of the experimental design for contextual fear conditioning, indicating the effect of PNN on the reconsolidation process. (A, Left) No significant differences were detected in the recall process after ChABC treatment for 24 h after training. (A, Right) The percentage freezing during the 3-min context exposure demonstrated significantly less freezing for the ChABC-treated animals during the reconsolidation process. Animals were treated with ChABC 24 h after training (n = 16 for PBS and n = 19 for ChABC). (B) Significant differences were detected during the remote reconsolidation process in ChABC-treated mice in ACC, and ChABC-treated mice at ACC, treated 3 wk after training (n = 18 for PBS and n = 20 for ChABC). (C, Top) Schematic drawing of the experimental design for contextual fear conditioning, indicating the effect of PNN on the reconsolidation process. (C, Left) Significant differences were detected in the reconsolidation process of recent memory after increasing PNN expression by Hapln1 before training (n = 22 for each group). (C, Right) Significant differences were detected during the remote reconsolidation process in Hapln1-treated mice at ACC (n = 22 for mCherry and n = 27 for Hapln1). ***P < 0.001, **P < 0.01, *P < 0.05 by 2-way repeated-measures ANOVA. All data are expressed as the mean ± SEM.