Fig. 6.
Distribution of viral RNA copies in blood and tissues of challenged ferrets. Six ferrets in each group were i.m. inoculated with 107.6 TCID50 of CB1/2014. At the time points indicated following lethal challenge, serum viral RNA copy numbers were determined by real-time PCR (A). At 5 d p.i., 3 ferrets in each group were killed, and tissues (lung, liver, kidney, spleen, and intestine) were assessed for viral RNA copy number by real-time PCR (B). The one-way ANOVA with Dunnett multiple comparison test was used to assess P values compared with each day p.i. of rHB29. *P < 0.0001 and **P = 0.0005 (A); *P = 0.001, **P = 0.0001, and ***P < 0.0001 (B). # No samples were collected as animals had died/been killed.