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Kinetic parameters calculated from the MM analysis shown in Fig. 3
| Parameter | Wild-type | Y684F | K685A | Y684F/K685A |
| Vmax, s−1 | 3.8 ± 0.1 | 4,6 ± 0.3 | 7.4 ± 0.5 | ND |
| Hill slope | 1.69 ± 0.06 | 1.21 ± 0.05 | 1.19 ± 0.03 | 0.96 ± 0.1 |
| Khalf, mM | 0.80 ± 0.02 | 2.66 ± 0.28 | 5.28 ± 0.50 | ND |
| Kprime, mM | 0.69 ± 0.04 | 3.25 ± 0.27 | 7.2 ± 0.52 | ND |
ND, not determined.
