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. 2019 Dec 17;7(1):e000829. doi: 10.1136/bmjdrc-2019-000829

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Epididymal adipose tissue (EpAT) histology shows no differences in immune cell infiltration. (A) Histological representative images of CD45⁺ staining in EpAT. (B) Flow cytometry analysis of CD45⁺ subsets in EpAT. (C) Quantification of CD45⁺, MAC3⁺ macrophages or CD3⁺ lymphocytes per 100 adipocytes. (D) mRNA expression of cellular markers and inflammatory genes in EpAT relative to HK genes. Data (B/C) are represented as HFD (n=14 and 15/group) and SFD (n=8/group) with mean±SD, (D) is presented as HFD (n=14 and 15/group) and SFD (n=8/group) with mean±SEM. HFD, high fat diet; SFD, standard fat diet.