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. 2019 Dec 16;116(52):26717–26726. doi: 10.1073/pnas.1915043116

Fig. 6.

Fig. 6.

GF SAMP receiving microbiota from FLO1-treated SAMP donors display attenuated DSS-induced colitis. (A) Study design. (B) Survival, (C) total body weight loss, and (D) total inflammatory scores in colons of GF SAMP recipients transplanted with microbiota from FLO1-treated donors compared to Dex- and vehicle-treated donors after DSS-induced colitis. (E) Representative photomicrographs of full-thickness H&E-stained colonic tissues show preservation of epithelial structure (black arrows) in FLO1 microbiota recipient mice versus the other 2 groups. (Scale bar: 100 µm.) (F) mRNA levels of IL-1α, TNFα, IL-1β, and COX2 in colonic tissues from GF SAMP recipients transplanted with either FLO1, Dex, or vehicle microbiota after DSS-induced colitis. Data are presented as relative fold difference compared with control mice (arbitrarily set as 1). All data are representative of 2 independent experiments and presented as median ± interquartile range, n = 8 for control and FLO1 groups and n = 4 for Dex group due to the high mortality rate. Statistical significance was determined by Gehan–Breslow–Wilcoxon test (C) and 1-way ANOVA followed by Dunn’s post hoc test (D and F); *P < 0.05 and **P < 0.02.