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. 2019 Dec 16;116(52):26734–26744. doi: 10.1073/pnas.1915571116

Fig. 1.

Fig. 1.

Characterization and scRNA-seq analysis of Aire−/− retinas. (A) Aire−/− retina fundus photographs (Left) and immunostaining of the corresponding cross-sections (Right) showing leukocytes (CD45; red) and nuclei (DAPI; blue). Representative examples of grade 1 to grade 4 uveoretinitis are shown. In this and other retina cross-sections, the following abbreviations are used: ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. (Scale bar, 50 μm.) (B) Fundus photographs of 4 Aire−/− retinas used for scRNA-seq. (C) UMAP plot showing different cell-type clusters in a merged dataset from duplicate samples of Aire−/− retinas (shown in B) and age- and sex-matched WT control retinas. (D) UMAP plot of immune cells in the merged dataset. (E) Stacked bar plots showing the proportions of nonmicroglial immune cell types in scRNA-seq datasets from Aire−/− and WT retinas. The numbers of immune cells in each dataset are indicated below.