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Gene expression in the embryonic mouse gut. Comparisons of FPKM from RNA-seq and Ct values after 40 cycles of qPCR of wild-type embryonic male mouse gut cDNA isolated at the developmental stages of E10.5, E12.5, and E14.5. We set FPKM = 5 as the value considered for a gene to be expressed; error bars are the SEMs for 3 biological replicates each.
