Fig. 2.

PSA signaling requires Dectin-1. (A) Western blot analysis of IκB degradation in macrophages at different time points after PSA stimulation. Relative density (r.d.) was measured with ImageJ software. (B) IL-10 liberation from CD4⁺ T cells cocultured with splenic DCs for 5 d in the presence of anti-CD3. Cocultures were either treated or not treated with PSA (50 μg/mL). IL-10 levels were measured by ELISA of culture supernatants and normalized by subtracting the medium control. Data represent average of 2 experiments. Error bars indicate SD values. Scores were assessed for statistical significance by t test. *P < 0.05. (C) EAE clinical disease scores of female C57BL/6J mice were measured daily for 30 d after induction by the MOG 3I5−55 peptide. Mice were treated with PBS or 50 μg of purified PSA. Depicted are the combined results of 2 independent experiments in which average disease scores were plotted.