Table II.
Detailed information on culture period (weeks) and total number of cultured testicular fragments analysed for each (pre)pubertal patient.
| Organ culture code | Age (years) | Time points of analysis (weeks) |
Total number of fragments (detailed in Supplementary Table SII) |
|
|---|---|---|---|---|
| Fresh | Cryopreserved-thawed | |||
| OCID22 | 6 | 2, 4, 5 | 0, 2, 4, 5 | 40 |
| OCID30 | 8.5 | 0, 1, 2, 4, 5 | 0, 1, 2, 4, 5 | 48 |
| OCID25 | 9 | 0, 2, 4 | 0, 2, 4 | 25 |
| OCID23 | 10 | 0 to 5 | 0, 1, 2, 4, 5 | 54 |
| OCID24 | 10 | 0 to 5 | 0, 1, 2, 4, 5 | 56 |
| OCID27 | 10.5 | 0, 2, 4, 5 | 0, 2, 4, 5 | 35 |
| OCID29 | 11 | 2, 4, 5 | 0, 2, 4, 5 | 38 |
| OCID26 | 12 | 1 to 5 | 1, 2, 4, 5 | 56 |
| OCID28 | 14 | 0 to 5 | 0 to 5 | 62 |
Depending on initial biopsy size, the number of cultured fragments and distribution throughout culture conditions and time points of analysis were variable between patients. Due to tissue scarcity, solely for one patient (OCID28) were all culture conditions evaluated at week 3.