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. Author manuscript; available in PMC: 2019 Dec 30.

Published in final edited form as: Biochemistry. 2016 Dec 8;55(50):6961–6968. doi: 10.1021/acs.biochem.6b00937

Table 2:

Thermodynamic parameters for guanidinium hydrochloride denaturation

	[denaturant]_1/2 (M)	$Δ G_{w}^{°}$ (kcal/mol)	m (kcal/mol·M⁻¹)
GndHCl (apo)	1.75	2.8 ± 0.095	1.6 ± 0.17
GndHCl (holo N → I)	0.74	0.77 ± 0.72	1.0 ± 0.59
GndHCl (holo I → U)	4.08	4.3 ± 0.37	1.1 ± 0.087