Fig 3. Identification of CWD prions in epididymides of white-tailed deer.
Epididymides from white tailed-deer were analyzed for the presence of CWD prions by PMCA. Samples and methods utilized for detection in this case were the same as listed for Figs 1 and 2. B+LN+: had PrPSc deposition in the brain stem and medial retropharyngeal lymph nodes (MRPLN) by immunohistochemistry (late pre-symptomatic); B- LN+: had PrPSc deposition only in the MRPLN (early pre-symptomatic); Neg: samples were non-detect for CWD in both the brain stem and the MRPLN. Numbers at the right represent molecular weight markers.