Fig 4. Modulation of PMCA efficiency by testes and epididymides homogenates.
Known amounts of brain-derived CWD prions (10−7–10−10 brain dilutions) were spiked in testis and epididymis homogenates from a CWD-negative white tailed-deer. Samples were processed in the same way as samples depicted in previous figures and tested in three PMCA rounds. Positive controls included samples spiked in PBS, or directly spiked in PMCA substrate (CWD (+) Ctrl). Negative controls comprised unseeded reactions (Ctrl1-4). Numbers at the right represent molecular weight markers. Dotted lines represent splicing of different membranes.