Fig 1. cPCH distributions are sensitive to the concentration, brightness, and diffusion of the fluorophores with a higher probability of photon counts near the nx = ny axis when τ < τd.
A) cPCH distributions at different concentrations and brightnesses at τ = τd (the diffusion time, τd, is 45 μs). B) cPCH distributions at different time shifts τ for a single species with a concentration of N = 25 molecules per confocal volume and a diffusion time of τd = 45 μs. The axial-to-radial beam waist ratio is s = 5, and the time-shifted channel is from the same spectral channel as the time 0 channel. C) Spatial cPCH distributions at different pixel separations (1, 5, and 25 horizontal pixels), using a diffusion coefficient of D = 100 μm2/s, a pixel size of δx = δy = 40 nm, a pixel dwell time of τp = 10 μs, and an image size of 512 × 512 pixels.