Fig 4. If both brightness and diffusion can be used to resolve the two species, cPCH can both resolve different species better than PCH and FCA and determine diffusion times.

A) Inference from 60 s of simulated data of two different species showing the ratio of the estimated parameters to the actual parameters when both brightness and diffusion differ between the two species. Parameters are: N₁ = 8, ϵ₁ = 48895 counts/s, and τ₁ = 87.85 μs for species 1; N₂ = 2, ϵ₂ = 146686 counts/s, and τ₂ = 439.24 μs for species 2. The bin time is T = 10 μs. B) Results of inference from 75 s of simulated data when only brightness substantially differs between the two species. Parameters are: N₁ = 8, ϵ₁ = 48895 counts/s, and τ₁ = 21.96 μs for species 1; N₂ = 5, ϵ₂ = 146686 counts/s, and τ₂ = 25.16 μs for species 2. The bin time is T = 10 μs.