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. 2019 Dec 30;14(12):e0226874. doi: 10.1371/journal.pone.0226874

Fig 1. Schematic experimental design showing the timeline of the experimental protocol.

Fig 1

Twenty-eight-day-old female rats were treated daily with s.c. injections of VCD or Oil for 15 days. The estrous cycle was assessed daily from day 56 after the onset of treatment until euthanasia. From 56 to 70 days after onset of treatment rats with irregular cycles were eliminated from the study. Rats were decapitated in diestrus at 70, 80, 90 and 100 ± 3 days after the onset of the treatment at 9–11 a.m. Trunk blood was collected for hormones measurements. PND, postnatal day; DAOT, days after the onset of the treatment.