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. 2019 Dec 30;14(12):e0226682. doi: 10.1371/journal.pone.0226682

Fig 2. mRNA quantitation of gpa genes in sporangiospores in gpa11 and gpa12 deletion mutants of M. circinelloides.

Fig 2

Total RNA was isolated from spores of the wild-type, Δgpa11, Δgpa12, and Δgpa11gpa12. The mRNA from each gpa gene was determined by qRT-PCR. Asterisks denote the phylogenetic group of the Gpa proteins in M. circinelloides (* I; **-II, and ***-III) [25]. Four independent experiments were performed under the same conditions. Different letters denote statistically significant differences (ANOVA, Fisher, p<0.05).