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. 2019 Dec 30;14(12):e0226682. doi: 10.1371/journal.pone.0226682

Fig 5. Sporangiospore germination of M. circinelloides gpa11 and gpa12 deletion mutants.

Fig 5

A) Percentage of germinated spores in aerobic conditions from each strain in YPG. Aerobic germinated cells were considered independent of hyphae length or number of mother cells. B) Relative expression of pkaR1, quantified by qRT-PCR using tfc-1 as reference gene from hyphae obtained from each strain grown at 2 and 4 h. ΔCT analysis was performed to compare the mRNA levels between strains. Spores from the different strains from M. circinelloides were inoculated into YPG media and incubated with constant shaking (150 rpm) for 3 h, and C) intracellular cAMP levels, and D) PKA activity were determined. E) Percentage of spores germinated under anaerobic conditions in YPG media. F) Relative expression of adh1 determined from yeast cells at 4 h of growth, ΔCT analysis was performed to compare mRNA levels between strains. Four independent experiments were performed for each condition. Statistically significant differences are indicated by different letters (ANOVA and Fisher’s tests; p ≤ 0.05).