Fig 9. Light chain somatic mutation is required for glycan reactivity of V3-glycan HIV-1 broadly neutralizing antibodies.

In vitro HIV-1 pseudovirus neutralization of JR-FL (filled bars) and kifunensine-treated (Man₉GlcNAc₂-enriched; open bars) JR-FL in the TZM-bl neutralization assay. Neutralization titers are shown in μg/mL as the concentration that inhibits 80% of virus replication (IC80). The antibody tested for neutralization is listed in the graph title. Variants of each antibody are listed on the x-axis. The unmutated common ancestors (UCA) of DH270 and PGT121 lack somatic mutation. For PGT128 the previously published germline reverted antibody (gl) was characterized [57]. PGT121 and PGT124 (reds) are from the same lineage and have the same UCA shown. PGT128 and PGT130 (greens) are clonally related and have the same germline (gl) antibody shown [20, 57].