Fig 1.

(A) SDS-PAGE and (B) Western immunoblotting of B. candidus venoms on a 12% separating gel with 5% stacking gel. Venoms were treated in reducing (R) or non-reducing buffer (N) prior to loading, electrophoresis, and stained with Coomassie Blue. T indicates B. candidus venom from Thailand, M indicates B. candidus venom from Peninsular Malaysia and I indicates B. candidus venom from Indonesia. (R) indicates venom treated with reducing sample buffer and (N) indicates venom treated with non-reducing sample buffer. Western immunoblotting of reduced B. candidus venoms incubated with monovalent B. candidus antivenom. m is molecular weight marker.