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. 2019 Dec 16;15(12):e1008513. doi: 10.1371/journal.pgen.1008513

Fig 2. Growth and translation in Rp+/- wing discs.

Fig 2

All panels show wing pouch regions of third-instar wing imaginal discs. Genotypes are as indicated below each figure; the font colors correspond to the labeling of the corresponding genotype (the genotype corresponding to the most brightly labeled cells is shown in white on a black background). A,B). Xrp1 protein levels were lower in RpS3+/- rpS12D97/D97 clones than in RpS3+/- rpS12D97/+ cells. Small RpS3+/- rpS12+/+ clones were detected rarely (eg arrow) C) RpS3+/- rpS12+/+ clones are rarely detected in RpS3+/- rpS12D97/+ wing discs, unlike the reciprocal RpS3+/- rpS12D97/D97 clones. D) In contrast to panel C, in the Xrp1+/- background RpS3+/- rpS12+/+ clones were recovered similarly to the reciprocal RpS3+/- rpS12D97/D97 clones. E,F). Overall translation was reduced in RpS17+/- cells, as was described previously [16]. G,H). Overall translation was not affected in RpS17+/- cells in rpS12D97/D97 wing discs. I,J) Translation in RpS18+/- cells is reduced compared to wt cells, as was described previously [16]. K,L). Further data related to this figure is shown in the S1 Fig. Genotypes: A-C) y w hsF; rpS12D97 FRT80B/P{arm-LacZ} FRT80B M(3)95A armLacZ. D) y w hsF; rpS12D97 FRT80B/P{arm-LacZ} FRT80B Xrp1m2-73 M(3)95A. E,F) y w hsF; RpS174 P{ubi-GFP} FRT80B/FRT80B. G,H) y w hsF; rpS12D97 RpS174 P{ubi-GFP} FRT80B/rpS12D97 FRT80B. I,J) y w hsF; FRT42D P{arm-LacZ} M(2)56i/FRT42D. K,L) y w hsF; FRT42D P{arm-LacZ} M(2)56i/FRT42D; rpS12D97 FRT80B/rpS12D97 FRT80B.