Fig 4. Reduced virus replication in the central nervous system after infection with the triple and quadruple mutant EEEV viruses.

CD-1 mice were infected with 10³ pfu of the EEEV mutants sc in each footpad. Tissues were harvested at 96 hours post infection. Virus replication in cortex (A), subcortex (B), cerebellum (C), and olfactory bulb (D). Data is log₁₀ transformed and represented as either RLU/LN or RLU per μg protein N = 8 mice, from 2 independent experiments. *P<0.5, **P<0.01, ***P<0.001, ****P<0.0001 one way analysis of variance test with corrections for multiple comparisons using the Holm-Sidak method comparing each mutant to WT. Box-and whisker plots represent min-max with bar representing the median value.