Figure 3. Gremlin1 regulates the adhesion, invasion and migration of GC cells. Cell adhesion assay (A) and transwell invasion assay (B) was performed to evaluate the impact of Gremlin1 on the adhesion and invasiveness of GC cell lines. Wounding assays were performed comparing HGC27SC and HGC27GREM1sh with semi-quantification of migration area using Image J (D) and representative images are shown for each cell lines (C). The wounding assay was employed to determine the migration of AGSpEF and AGSGREM1exp followed by semi-quantification of migration area using Image J (E and F). Three independent experiments were performed. **Represent p<0.01, ***represent p<0.001.