Figure 4. GREM1 and EMT in GC. (A) PCR were performed to test the expression of EMT markers in the GREM1knockdown HGC27 cells and Gremlin1 overexpression AGS cells. Correlation between GREM1and EMT markers in the TCGA-GC cohort was analysed using Spearman tests, results shown as a heatmap (B) and scatter plots of significantly related EMT markers (C-H).