KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-beta3-tubulin | Millipore Sigma | T8660; RRID:AB_477590 |
| Rabbit anti-TPP1 | Abcam | Ab96498, RRID; AB_10680725 |
| Mouse αnti-Myc, clone 4A6 | EMD Millipore | 05724, RRID; AB_309938 |
| Rabbit monoclonal anti-Isl1 | Abcam | Ab109517, RRID; AB_10866454 |
| Mouse anti-MAP2A,2B | EMD Millipore | MAB378, RRID; AB_94967 |
| Bacterial and Virus Strains | ||
| CMV-mitoDSred LV | Orian Shririhai, UCLA | N/A |
| Synapsin-mitoDSred LV | This study | N/A |
| pTet-O-Ngn2-puro | Zhang et al., 2013 | https://www.addgene.org/52047/ |
| Tet-O-FUW-EGFP | Vierbuchen et al., 2010 | https://www.addgene.org/30130/ |
| FUW-M2rtTA | Hockemeyer et al., 2008 | https://www.addgene.org/20342/ |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Biomol ICCBL – 2012 library (480) | ICCB-Longwood | https://iccb.med.harvard.edu/biomol-iccb-l-known-bioactives-2012 |
| Selleck Bioactive compound library (1,902) | ICCB-Longwood | https://iccb.med.harvard.edu/selleck-bioactive-compound-library |
| Calcimycin | Millipore Sigma | A23187 |
| Latrunculin B | Tocris | Cat # 3974 |
| Latrunculin A | Tocris | Cat # 3973 |
| Cytochalasin B | Tocris | Cat # 5474 |
| Ala-Ala-Phe-CMK | Enzo | BML-PI123-0025 |
| BMS-754807 | Selleck Chemicals | Cat # S1124 |
| Hesperadin | Selleck Chemicals | Cat # S1529 |
| TAK-901 | Selleck Chemicals | Cat # S2718 |
| Barasertib | Selleck Chemicals | Cat # S1147 |
| Paclitaxel | Tocris | Cat# 1097 |
| 24(S)-Hydroxycholesterol | Millipore Sigma | Cat # SML1648 |
| Butabindide oxalate | Tocris | Cat# 1323 |
| Carfilzomib (PR-171) | Selleck Chemicals | Cat # S2853 |
| Y-27632(ROCK inhibitor) | Cayman Chemicals | Cat# 10005583 |
| Tetramethylrhodamine, methyl ester | ThermoFisher | Cat #T668 |
| Deposited Data | ||
| Primary screen data were deposited at GitHub | This study | https://github.com/ThomasSchwarzLab/ScreeningData_Shlevkov_CellReports_2019 |
| Experimental Models: Cell Lines | ||
| HEK293T | ATCC | ATCC CRL-3216, RRID:CVCL_0063 |
| GON0515-03 #5 iPSC line | This study | N/A |
| 39b SOD+/A4V iPSC line | Wainger et al., 2014 | N/A |
| 39b SOD+/+ iPSC line | Wainger et al., 2014 | N/A |
| Experimental Models: Organisms/Strains | ||
| Syntaphilin KO mouse | Kang et al., 2008 | N/A |
| Oligonucleotides | ||
| Primer AurkB K106R (forward): GTGGCGTCAGGGTCCTCTTG | This study | N/A |
| Primer AurkB K106R (reverse): GATGAAATGGCTTTTCTTCTCCC | This study | N/A |
| Primer synapsin-mitoDSred Lenti (forward): GCCTGAGAGCGCAGTAGCGCTACCGGACTCAGA | This study | N/A |
| Primers for synapsin-mitoDSred Lenti (reverse): CCGCTAGCTTCGAAGATTATGATCTAGAGTCGCGGC | This study | N/A |
| Recombinant DNA | ||
| Mito-DsRed (pDsRed2-Mito) | G. Hajnoczky, Thomas Jefferson University | N/A |
| GFP-rab7 WT | Choudhury et al., 2002 | Addgene #12605 |
| pLV-CMV-mitoDSred | This study | N/A |
| pLV-Synapsin-mitoDSred | This study | N/A |
| pGFP-C-shLenti-Tpp1 (rat) | OriGene | TL711322 |
| pCMV6-Myc-DDK-Tpp1 (human) | OriGene | RC204471 |
| pGFP-C-shLenti-Tpp2 (rat) | OriGene | TL711260 |
| pGFP-C-shLenti-Aurkb (rat) | OriGene | TL712117 |
| pCMV6-Myc-DDK-Aurkb (human) | OriGene | RC210288 |
| pCMV6-Myc-DDH-AurkBK106R (kinase dead) | This study | N/A |
| pGFP-C-shLenti-Aurka (rat) | OriGene | TL320538 |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| MATLAB® | MathWorks | https://www.mathworks.com |
| CellProfiler | Carpenter et al., 2006 | https://cellprofiler.org |
| R | R Core Team, 2014 | https://www.R-project.org |
| GraphPad Prism v6.0 | GraphPad Software | https://www.graphpad.com |
| Other | ||
| Cell Profiler pipeline for motility analysis and PATHS: MATLAB-based code for processing of Cell Profiler data for motility analysis, deposited at GitHub | This study | https://github.com/ThomasSchwarzLab/ArrayScanCodes |