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. Author manuscript; available in PMC: 2019 Dec 30.
Published in final edited form as: Cell Rep. 2019 Dec 3;29(10):3087–3100.e7. doi: 10.1016/j.celrep.2019.11.013

Figure 7. C1q Mediates Sensory-Motor Synaptic Refinement during Normal Development.

Figure 7.

(A) Righting times in WT (n = 10) and C1q−/− (n = 18) mice during the first 12 postnatal days. ***p < 0.001; t test for the individual ages.

(B) Schematic illustration for retrograde labeling of TA MNs and their proprioceptive synapses with CTb-555 (red) and gastrocnemius MNs only with fluorescinated dextran (green). The dotted red line indicates inappropriate TA synapses onto gastrocnemius MNs.

(C) TA and gastrocnemius MNs labeled with CTb-555 and fluorescinated dextran correspondingly.

(D) Single optical planes of confocal images with the tracers fluorescinated dextran (green), CTb-555 (red) and VGluT1 (blue) immunoreactivity from gastrocnemius MNs in a WT and a C1−/− mouse at P4. Inset shows a TA-originating proprioceptive synapse (arrow) on a gastrocnemius MN.

(E) Percentage of MNs receiving inappropriate synapses in WT (n = 3) and C1q−/− mice (n = 4). *p < 0.05; t test.