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. 2019 Dec 3;8:e48479. doi: 10.7554/eLife.48479

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© 2019, Ye et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 7. — (A–D) Representative image of the EEC calcium response to glucose (A, B) and palmitate (C, D) stimulation in 6 hr high fat (HF) fed and 6 hr HF fed with 0.5 mM orlistat treated Tg(neurod1:Gcamp6f) zebrafish larvae. (E, F) Quantification of the EEC calcium response to glucose and palmitate stimulation in 6 hr HF fed and 6 hr HF fed with 0.5 mM orlistat treated zebrafish larvae. (G–H) Confocal projection of Tg(neurod1:RFP); Tg(gata5:lifeAct-EGFP) zebrafish intestine in 10 hr HF fed larvae (G) and 10 hr HF fed with 0.1 mM orlistat treated larvae (H). (G’–H’) Zoom view of EECs in indicated conditions. The yellow arrows in G’ and H’ indicate the EECs’ apical extensions. (I–J) Confocal images of Tg(neurod1:RFP); Tg(ef1α:xbp1δ-GFP) zebrafish intestine in 6 hr HF fed larvae (I) and 6 hr HF fed with 0.5 mM orlistat treated larvae (J). (I’–J’) Zoom view of EECs in indicated conditions. Yellow arrows in I’ indicate the EECs with activated xbp1 in HF fed condition. (K–M) Confocal images of Tg(neurod1:RFP); Tg(NFΚB:EGFP) zebrafish intestine in 10 hr HF fed larvae (K), 10 hr HF fed larvae treated with 0.1 mM orlistat (L) and 10 hr HF fed larvae treated with 0.5 mM TUDCA (M). Yellow arrows indicate neurod1:RFP+ EECs co-labeled with the NFΚB reporter. (N) Quantification of the EEC morphology score in control, 10 hr HF fed and 10 hr HF fed with 0.1 mM orlistat treated larvae represented in G and H. (O) Quantification of s-xbp1+ EEC (%) in 6 hr HF fed larvae and 6 hr HF fed larvae treated with 0.5 mM orlistat represented in J and K. (P) Quantification of NF-κB+ EECs in control, 10 hr HF fed, 10 hr HF fed with 0.1 mM Orlistat and 10 hr HF fed with 0.5 mM TUDCA treated zebrafish larvae represented in K-M. Student t-test was performed in E, F, O and one-way ANOVA with post-hoc Tukey test was used in N, P for statistical analysis. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Figure 7—figure supplement 1. — (A–D) Representative image of the EEC calcium response to glucose (A, B) and palmitate (C, D) stimulation in 6 hr high fat (HF) fed and 6 hr HF fed with 0.5 mM orlistat treated Tg(neurod1:Gcamp6f) zebrafish larvae. (E, F) Quantification of the EEC calcium response to glucose and palmitate stimulation in 6 hr HF fed and 6 hr HF fed with 0.5 mM orlistat treated zebrafish larvae. (G–H) Confocal projection of Tg(neurod1:RFP); Tg(gata5:lifeAct-EGFP) zebrafish intestine in 10 hr HF fed larvae (G) and 10 hr HF fed with 0.1 mM orlistat treated larvae (H). (G’–H’) Zoom view of EECs in indicated conditions. The yellow arrows in G’ and H’ indicate the EECs’ apical extensions. (I–J) Confocal images of Tg(neurod1:RFP); Tg(ef1α:xbp1δ-GFP) zebrafish intestine in 6 hr HF fed larvae (I) and 6 hr HF fed with 0.5 mM orlistat treated larvae (J). (I’–J’) Zoom view of EECs in indicated conditions. Yellow arrows in I’ indicate the EECs with activated xbp1 in HF fed condition. (K–M) Confocal images of Tg(neurod1:RFP); Tg(NFΚB:EGFP) zebrafish intestine in 10 hr HF fed larvae (K), 10 hr HF fed larvae treated with 0.1 mM orlistat (L) and 10 hr HF fed larvae treated with 0.5 mM TUDCA (M). Yellow arrows indicate neurod1:RFP+ EECs co-labeled with the NFΚB reporter. (N) Quantification of the EEC morphology score in control, 10 hr HF fed and 10 hr HF fed with 0.1 mM orlistat treated larvae represented in G and H. (O) Quantification of s-xbp1+ EEC (%) in 6 hr HF fed larvae and 6 hr HF fed larvae treated with 0.5 mM orlistat represented in J and K. (P) Quantification of NF-κB+ EECs in control, 10 hr HF fed, 10 hr HF fed with 0.1 mM Orlistat and 10 hr HF fed with 0.5 mM TUDCA treated zebrafish larvae represented in K-M. Student t-test was performed in E, F, O and one-way ANOVA with post-hoc Tukey test was used in N, P for statistical analysis. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.