Author response image 1. CM-H₂DCFA (a molecular probe indicating ROS levels) shows highest activity in the gut lumen, gall bladder, gut epithelium, and nephric duct in 6dpf conventionally-raised zebrafish larvae (left panel).

Platereader measurements of CM-H₂DCFA levels in GF and CV zebrafish larvae with or without PMA induction, show no significant differences (right panel).