KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| ERL strain, E. coli | Robert Sauer Lab (MIT) | N/A |
| BL21 Star (DE3) | ThermoFisher | C6010-03 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BirA biotin-protein ligase standard reaction kit | Avidity | BirA-500 |
| HaloTag Ligand Amine O4 | Promega | P6741 |
| (3-Aminopropyl) trimethoxysilane | Sigma-Aldrich | 281778 |
| Repel-Silane | GE Healthcare | 17-1332-01 |
| Glutaraldehyde solution (Grade I, 25% in H2O) | Sigma-Aldrich | G5882 |
| Tris(2-carboxyethyl)phosphine hydrochloride | Sigma-Aldrich | C4706 |
| L-Ascorbic Acid | Sigma Aldrich | A7506 |
| Hydrogen Peroxide 30% in H2O | Fisher Scientific | H325 |
| Bovine Serum Albumin, sulfhydryl blocked | Lee Biosolutions | 100-10SB |
| Recombinant DNA | ||
| Halo-SpyTag | This study | N/A |
| SpyCatcher-I278(32C/75C) | This study | N/A |
| pFN18A HaloTag T7 Flexi Vector | Promega | G2751 |
| pQE80L vector | QIAGEN | 32932 |
| Software and Algorithms | ||
| Magnetic Tweezers Software (MT 2.2) | Fernandez Lab | http://fernandezlab.biologycolumbia.edu/downloads |
| Other | ||
| Dynabeads M-270 Streptavidin | ThermoFisher | 65305 |
| Amino-polystyrene particles (2.5–2.9 micron) | Spherotech | AP-25-10 |
| xiQ CMOS camera | Ximea | MQ013MG-ON |
| PIFOC objective nano-positioner | Physik Instrumente | P-725.CDD |
| Collimated LED lamp | Thorlabs | MCWHL5 |
| N52 magnets 3/16’’ diameter | K&J Magnetics | D33-N52 |
| Analog filter module | Stanford Research Instruments | SIM965 |