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. 2019 Dec 30;9:20328. doi: 10.1038/s41598-019-56416-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elevated frequencies, but poor activity of ILC3 in the small intestine of BALB/c mice. (a) Representative FACS plots of CD4 and RORγt expression by small intestinal cells in G. muris-infected BALB/c and C57BL/6 mice 14 days post-infection (left panel) and of IL-17A expression by CD4^-RORγt⁺ cells in naïve and infected BALB/c and C57BL/6 mice (right panel). The histogram plot reports CD90 expression by CD4^-RORγt⁺ cells. (b) Frequencies of CD4^-RORγt⁺ cells in siLP cells. (c) Frequencies of IL-17A⁺ cells in CD4^-RORγt⁺ cells after PMA/ionomycin stimulation. Data are pooled from three (b) or two (c) independent experiments with n = 3–5/group. Statistical analysis was done using Kruskal-Wallis test combined with Dunn’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001. Further information on the phenotype of CD4^-RORγt⁺ cells is available in Supplementary Fig. 2d–g.