Figure 4.

Clustering example biological data using the transfer learning approach. t-SNE plots of mouse colonic sensory neurons: colour refers to clusters derived from the three approaches (A) TargetCluster, using only data from Hockley et al.²¹ to assign clusters. (D) ConcatenateCluster, using a concatenation of data from Hockley et al.²¹ and Usoskin et al.²⁰ (mouse sensory neurons) to assign clusters. (G) TransferCluster, using the novel transfer learning approach with Usoskin et al.²⁰ as Source and Hockley et al.²¹ as Target) and shape refers to spinal segment from which the neuron was isolated (triangle, TL (thoracolumbar); circle, LS (lumbosacral)). In G, clusters 1 and 7 (black dashed boxes), cluster 2 (blue dashed box) and cluster 4 (green dashed box) represent biologically distinct groups of cells with differing sensory functions. This is exemplified by the cluster-specific expression of specific genes by cluster 7 (B), Mrgprd), 1 (C, P2ry1), 2 (E, Ntm) and 4 (F, Spp1). Colour scheme represents expression level [log(TPM)].