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. 2019 Dec 30;9:20304. doi: 10.1038/s41598-019-56835-8

Figure 6.

Figure 6

mTOR, but not PPARγ, pathway inhibition affects COX-2 expression induced by ASCcm in macrophage. Macrophages were pre-treated with mTOR inhibitor (A) rapamycin (20 nM) or PPARγ inhibitor (B) GW9662 (10 nM), 30 min before treatment with ASCcm (50%) for 24 h. Analysis of COX-2 expression was performed in total cell lysates of macrophages by western blot. β-Actin levels were used for control of protein loading. Data are expressed as mean ± SEM from five independent experiments. *p < 0.05 versus non treated cells (CTRL) and #p < 0.05 versus ASCcm treated cells.