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. 2019 Dec 30;9:20369. doi: 10.1038/s41598-019-56814-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative single channel glycerol and urea permeabilities of AQPs 3, 9 and 10. (A) Representative calcein fluorescence quenching curves for human AQPs 3, 9 and 10 for glycerol and urea permeability. (B) Average cell swelling rate constants from fits to calcein fluorescence timeseries data. (C) Surface expression of GFP-tagged AQPs 3, 9 and 10 in HEK293 cells measured by cell surface biotinylation followed by ELISA with an anti-GFP antibody. (D) Relative single channel solute permeabilities of AQPs 3, 9 and 10 calculated by normalizing the cell swelling rate constant to surface expression and then to AQP3 glycerol permeability. (E) Ratio of urea permeability to glycerol permeability for AQPs 3, 9 and 10. This figure is reproduced from P. Kitchen PhD thesis³⁵.