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. 2019 Dec 30;9:20365. doi: 10.1038/s41598-019-56733-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neighbour-joining tree based on 16S rRNA gene sequences showing the relationship between cloned sequences from Phormia regina adults and sequences of other closely related bacterial species. Clone “16S CLONE C8” originated from sample (Fi12) that contained 92% of sequences assigned to the genus Dysgonomonas. Aquifex pyrophilus was used as an outgroup. The sequences were aligned using the ClustalW algorithm. Bootstrap values based on 500 replications, are given at the branching nodes. Bar represents 0.05 substitutions per nucleotide position.