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. 2019 Dec 30;9:20332. doi: 10.1038/s41598-019-56993-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEC-MALS, AUC and native MS analysis of C2_TM_CT wild-type and mutants. (a) SEC-MALS of C2_TM_CT. (b) SEC-MALS of C2_TM_CT_M1. (c) SEC-MALS of C2_TM_CT_M2. Chromatograms show the detector readings of the LS and UV in red and black, respectively. Scales for LS and UV detectors are shown in the left-hand axis; and calculated molecular mass on the right-hand axis. (d) AUC (sedimentation velocity profiles) of C2_TM_CT and mutants. (e) Native mass spectra of C2_TM_CT wild-type and the mutant variants (1 & 2) solubilized in DDM micelles with the 2D drift plot of the wild-type in the top panel. In the case of overlapping oligomeric species, the drift plot can help to identify the correct oligomeric states that are represented by the same m/z peak. Monomeric and oligomeric species including their charge states are assigned accordingly. Corresponding peaks of C2_TM_CT_M2 are slightly shifted to lower m/z values due to the deletion of six amino acids.