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. 2019 Dec 30;9:20341. doi: 10.1038/s41598-019-56456-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ChIP-qPCR to test in vivo interaction of promoters (including 5’UTR) with AtOXS2 in 10-d-old seedlings from oxs2-1 and oxs2-1 (AtOXS2-FLAG) treated with 150 mM NaCl. Promoter or segments tested are labeled F1-F12. CP (crossing point) value of immuno-precipitated DNA fractions with α-FLAG or no antibody control (NoAb) normalized to CP value of input DNA fractions for the same qPCR assay. Y axis is the ChIP signals calculated as the enrichment relative to the no-antibody control (No Ab). Error bars indicate ± SD from three independent experiments. P value of Student’s t test: oxs2-1 (AtOXS2-FLAG) compared with oxs2-1. *P < 0.05; ***P < 0.001.